Strain Data Sheet
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC00897  
Type Targeted Mutation CongenicCartagena   wks
Species Mus musculus  
Strain name B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc  
Former Common name AID-KO C57BL/6 N10, B6-AID KO#N10, B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line  
Background strain No Data  
 1 Appearance Black  
Genotype a/a B/B C/C  
Strain development Developed by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into TT2 ES cells derived from (C57BL/6 x CBA)F1. The mutant mice were backcrossed to C57BL/6N.  
Strain description AID (Aicda, activation-induced cytidine deaminase) knockout mice. Exon 2 and part of exon 3 were replaced with a neomycin resistance cassette. Aid, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation. AID deficiency caused a complete defect in class switching and showed a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. Accumulation of IgM positive B cell is remarkable in the gut lamina propria. Furthermore, it is suggested that a mutation of the Aid gene was a cause of the hereditary immune deficiency disorder, type-2 hyper-IgM syndrome (HIGM2) in human. As well as class switch recombination, it was also indicated that somatic hypermutation of an immunoglobulin variable region gene and gene conversion do not occur at all in the Aid deficiency mice. Aid gene is a molecule serving as a central role in antibody gene variety acquisition mechanism of B cell.  
Colony maintenance Homozygote x Homozygote. Homozygous mutant mice are viable and fertile.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Aicda  
Symbol name Activation-induced cytidine deaminase  
Chromosome 6  
Common name AID  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 6  
Common name neo; neomycin;  
Symbol description No Data  
References Cell. 2000 Sep 1;102(5):553-63.  
Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.
Research applications Cre/loxP system,
Immunology and Inflammation Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Cell 102, 553-563 (2000).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.  
Additional information
 1 Honjo Lab HP  
 2 Mouse of the Month Dec 2005
Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University)  Honjo, Tasuku 
Strain Status /
(Expected delivery)

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