Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC00904  
Type Targeted Mutation CongenicCartagena  
Species Mus musculus  
Strain name C.129S2-Pdcd1<tm1Hon>/HonRbrc (N12)  
Former Common name PD-1-KO-N12 (BALB/c)  
H-2 Haplotype No Data  
ES cell line D3 [129S2/SvPas]  
Donor strain 129S2/SvPas via D3 ES cell line  
Background strain BALB/cCrSlc  
Appearance
 1 Appearance albino  
Genotype A/A b/b c/c  
Strain development Developed by Dr. Tasuku Honjo, Kyoto University.  
Strain description The PD1 (programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, and plays a role in the negative control of proliferation, differentiation and class switching of B cells. Homozygous mutant mice exhibit abnormalities in leukopoiesis and the immune system which vary considerably depending on the genetic background. The homozygous mutant mice with BALB/c background exhibit autoimmune dilated cardiomyopathy. The mutant mice of the BALB/c-PD1 (N10 line) show severe dilated cardiomyopathy and start to die as early as 5 weeks of age. Meanwhile, the BALB/c-PD1 (N12) line mutant mice exhibit less severe dilated cardiomyopathy and survive longer than N10 line.  
Colony maintenance Sibling Mating (Homozygote x Homozygote)
Homozygous mutant mice are fertile and viable.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Pdcd1  
Symbol name programmed cell death 1  
Chromosome 1  
Common name Ly101, PD-1, Pdc1  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 1  
Common name neo; neomycin;  
Symbol description No Data  
References Immunity. 1999 Aug;11(2):141-51.
Int Immunol. 1998 Oct;10(10):1563-72.
Science. 2001 Jan 12;291(5502):319-22.  
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Research applications Immunology and Inflammation Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science 291, 319-322 (2001).
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.  
Additional information
 1 Honjo Lab HP  
 2 Mouse of the Month Mar 2005  
 3 Genotyping protocol <PCR>  
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University)  Honjo, Tasuku 
Strain Status /
Availability
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