Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 19, 2019
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  Mouse Marking
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RBRC No. RBRC00980  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6C3F1-Tg(CAG-EGFP)CX-FM038Osb  
Former Common name 152A; B6C3F1B6C3F1-TgN(act-EGFP)OsbCX-FM038  
H-2 Haplotype No Data  
Background strain Slc:B6C3F1  
Appearance
 1 Appearance black or agouti  
Genotype a/a B/B C/C or A/? B/B C/C  
Strain development Developed by Dr. Masaru Okabe at Osaka University in 1997. The CAG-GFP plasmids were injected into the pronuclei of B6C3F2 fertilized eggs.  
Strain description The CAG-EGFP transgene is located on X chromosome. This strain is useful for detection of cell fate in transplantation and developmental studies.  
Colony maintenance Sibling Mating  
Health Report
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 1 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome X  
Common name No Data  
Symbol description No Data  
References Curr Biol. 2006 Jan 24;16(2):166-72.
FEBS Lett. 1997 May 5;407(3):313-9.
Genomics. 2002 Dec;80(6):564-74.  
Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the x-linked gene, rhox5/pem, at preimplantation stages.
Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the x-linked gene, rhox5/pem, at preimplantation stages.
Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the x-linked gene, rhox5/pem, at preimplantation stages.
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics, 80, 564-574 (2002). Curr Biol., 16(2):166-172 (2006).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Okabe, Masaru (Osaka university)  Okabe, Masaru 
Strain Status /
Availability
(Expected delivery)

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