Strain Information | |
---|---|
Image | |
BRC No. | RBRC00986 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6C3F1-Tg(CAG-EGFP)CX-FM50Osb |
Former Common name | B6C3F1B6C3F1 TgN(act-EGFP)OsbCX-FM050 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Masaru Okabe at Osaka University in 1997. The CAG-GFP plasmids were injected into the pronuclei of B6C3F2 fertilized eggs. |
Strain description | Both homozygous and hemizygous transgenic mice express GFP in all over the body. The transgene is located in X chromosome. This strain is useful for detection of cell fate in transplantation and developmental studies. |
Colony maintenance | Sibling Mating (Homozygote x Hemizygote) |
References | FEBS Lett., 407, 313-319 (1997). 9175875 Genomics, 80, 564-574 (2002). 12504848 Proc. Natl. Acad. Sci. USA, 102(11), 4039-4044 (2005). 15746241 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPGreen Fluorescent Protein (Aequorea victoria)XEGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Ordering Information | |
---|---|
Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genomics, 80, 564-574 (2002). Proc. Natl. Acad. Sci. USA, 102(11), 4039-4044 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masaru Okabe (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |