Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC00995  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6.129P2-Il5ra<tm1Ktk>/KtkRbrc  
Former Common name C57BL/6 IL-5R alpha -/-  
H-2 Haplotype No Data  
ES cell line E14.1 [129P2/OlaHsd]  
Donor strain 129P2/OlaHsd via E14.1 ES cell line  
Background strain C57BL/6NCrSlc  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Kiyoshi Takatsu, Institute of Medical Science in 1996. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6CrSlc.  
Strain description L-5Ralpha gene knockout mice. A translational stop codon was introduced into exon 5 of the Il5ra and the exon 6 was replaced with a neo cassette.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Il5ra  
Symbol name interleukin 5 receptor, alpha  
Chromosome 6  
Common name CD125, CDw125, IL-5 receptor alpha chain, Il5r, IL-5Rα  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 6  
Common name neo; neomycin;  
Symbol description No Data  
References Immunity. 1996 May;4(5):483-94.
Immunology. 2001 Mar;102(3):289-300.  
Defective B-1 cell development and impaired immunity against Angiostrongylus cantonensis in IL-5R alpha-deficient mice.
Defective B-1 cell development and impaired immunity against Angiostrongylus cantonensis in IL-5R alpha-deficient mice.
Research applications Immunology and Inflammation Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Immunity 4, 483-494 (1996).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The depositor should be a co-author in the articles when the users publish their data using these mice for 1 year after provided.
Users should contact the depositor in the case of application for any patents with the results from these mice.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Takatsu, Kiyoshi (The Institute of Medical Science, The University of Tokyo)  Takatsu, Kiyoshi 
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