Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC01120  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6.Cg-Agt<tm1Afu>/AfuRbrc  
Former Common name AGT-KO (BL/6)  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line  
Background strain C57BL/6JJcl  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Dr. Tanimoto K., University of Tsukuba in 2004. C57BL/6 background.  
Strain description B6.B6CB-Agt<tm1Afu>. Homozygous mutant mice do not produce angiotensinogen in the liver, resulting in the complete loss of plasma immunoreactive angiotensin I. The systolic blood pressure of the homozygous mutant is lower than wild-type, demonstrates an indispensable role for the renin-angiotensin system in maintaining blood pressure.  
Colony maintenance Sibling Mating (Heterozygote x Wild-type)
Homozygous mutant mice of C57BL/6 background show a low survivability. About 70 percent of homozygous mice  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Agt  
Symbol name Angiotensinogen  
Chromosome 8  
Common name Aogen  
Symbol description No Data  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 8  
Common name neo; neomycin;  
Symbol description No Data  
References J Biol Chem. 1994 Dec 16;269(50):31334-7.  
Angiotensinogen-deficient mice with hypotension.
Research applications Cell Biology Research,
Hematological Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Biol. Chem., 269, 31334-31337 (1994).  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Fukamizu, Akiyoshi (University of Tsukuba)  Fukamizu, Akiyoshi 
Strain Status /
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