Strain Data Sheet
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Data update: Aug 20, 2019
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RBRC No. RBRC01148  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name ICR.Cg-Nanos1<tm1Ysa>/YsaRbrc  
Former Common name nanos1 Knockout mice  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line  
Background strain ICR MCH  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Dr. Yumiko Saga, Natinal Institute of Genetics in 2000. LacZ and PGK-neo were inserted just before the translational start site of the nanos1 gene. The neo cassette was excised by crossing with CAG-Cre mice. Jcl:MCH (ICR) background.  
Strain description ICR.Cg-Nanos1<tm1Ysa>/YsaRbrc. Nanos1, nanos homolog 1 (Dorosophila) is a nanos-related gene. Nanos encodes a RNA-binding protein containing a zinc-finger motif required for abdomen formation in Dorosophila. The nanos1 deficient mice are fertile and they show no detectable abnormality. Beta-gal activity indicates the nanos1 gene expressed in adult brain and gonad. This strain is useful for elucidation of the role of the nanos and the related genes.  
Colony maintenance Sibling Mating (Homozygote x Homozygote)  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Nanos1  
Symbol name nanos homolog 1 (Drosophila)  
Chromosome 19  
Common name No Data  
Symbol description No Data  
 2 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 19  
Common name No Data  
Symbol description E. coli beta galactosidase  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 19  
Common name No Data  
Symbol description No Data  
References Mech Dev. 2003 Jun;120(6):721-31.  
nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development.
Research applications Developmental Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mechanisms of Development, 120, 721-731 (2003).  
Additional information
 1 Saga Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Saga, Yumiko (National Institute of Genetics)  Saga, Yumiko 
Strain Status /
Availability
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