Strain Data Sheet
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Data update: May 20, 2019
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RBRC No. RBRC01198  
Type Transgene CongenicCartagena  
Species Mus musculus  
Strain name 129.Cg-Tg(GOF18dPE/EGFP)48Ymat  
Former Common name 129;B6D2-Tg(GOF18δPE/EGFP)48, GOF18-delta PE-GFP.129  
H-2 Haplotype No Data  
Donor strain B6D2F1  
Background strain 129/Sv (hemizygote x 129/Sv)  
Appearance
 1 Appearance White-bellied gray  
Genotype No Data  
Strain development Developed by Hans R. Shoeler University of Pennsylvania and Yasuhisa Mastui, Research Institute Oasaka Medical Center of Maternal and Child Health in 1997. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mutant mice were backcrossed to C57BL/6 over 3 times (RBRC00868 and RBRC01198). Backcrossed 129 for 7 time (RBRC01198) (by Atsuo Ogura, RIKEN BioResrouce center).  
Strain description Oct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) (Dev Growth Differ 1999). Oct-4 gene expresses in undifferenciated spermatogonia at prepubertal stages. This strain is useful to detect primordial germ cells with green fluorescence protein by the PGC-specific promoter. Tg line including the proximal enhancer (PE) of GOF18 (RBRC00868), Tg line lacking the proximal enhancer (PE) (RBRC00869 and RBRC01198).  
Colony maintenance Carrier x Noncarrier [or Crossing to 129<+Ter>/SvJcl]  
Health Report No Data  
Gene Details
Promoter Oct 3 promoter  
 1 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Dev Growth Differ. 1999 Dec;41(6):675-84.  

Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Purpose of study should be clarified.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP  
Depositor Ogura, Atsuo (RIKEN, BioResource Center)  Ogura, Atsuo 
Strain Status /
Availability
(Expected delivery)

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