Strain Data Sheet
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Data update: Jul 19, 2019
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RBRC No. RBRC01230  
Type Targeted Mutation and Chromosomal Aberration and F2 HybridCartagena  
Species Mus musculus  
Strain name B6;129-Foxh1<tm1Hmd>/HmdRbrc  
Former Common name B6;129-FoxH1flox/flox, FASTflox/flox, B6;129-FoxH1tm2Hh(B6;129-FoxH1flox/flox,FASTflox/flox)Knockout mice  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Donor strain (129X1/SvJ x 129S1/Sv)F1-Kitl+ via R1 ES cell line  
Background strain No Data  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
 2 Appearance white-bellied agouti  
Genotype A/? B/B C/C  
Strain development Developed by Drs. Masamichi Yamamoto and Hiroshi Hamada at Osaka University in 2001. C57BL/6Cr and (129X1/SvJ x 129S1/Sv)F1-Kitl+ mixed background.  
Strain description The transcription factor Foxh1 (horkhead box H1) gene mediates signaling by transforming growth factor-β, Activin, and Nodal. Foxh1 protein interacts with phospho-Smad2 to regulate specific downstream targets of these signals. Mutation in Foxh1 results in impaired orientation of anterior-posterior axis and various defects of anterior streak and node. The flox allele of this strain was generated by Flp-mediated deletion of the neo cassette located between frt sites from the original targeting vector which was designed to insert a combined loxP site and loxP-frt-neo-frt cassette. Conditional Foxh1 deficient mice can be generated by crossing with tissue-specific Cre mice. This strain is useful for the study of Foxh1 gene in vivo.  
Colony maintenance Sibling Mating (Homozygote x Homozygote)
Homozygote mutant mice are normal and fertile.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol FoxH1  
Symbol name forkhead box H1  
Chromosome 15  
Common name FAST  
Symbol description No Data  
 2 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 15  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 15  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 15  
Common name No Data  
Symbol description No Data  
References Genes Dev. 2001 May 15;15(10):1242-56.  
The transcription factor FoxH1 (FAST) mediates Nodal signaling during anterior-posterior patterning and node formation in the mouse.
Research applications Cre/loxP system,
Developmental Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on
use of it from the DEPOSITOR.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology)  Hamada, Hiroshi 
Strain Status /
Availability
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