Strain Data Sheet
RBRC01253
Strain Information | |
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| Image | |
| BRC No. | RBRC01253 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129S4-Gopc<tm1.1Tno>/Rbrc |
| Former Common name | GOPC Knockout mouse |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Drs. Ryoji Yao and Tetsuo Noda, Japanese Foundation for Cancer Research Cancer Institute in 1999. Genomic sequence containing 2, 2b, 3 and 4 of Gopc was replaced by the insertion of a LacZ and a floxed neo cassette. The floxed neo cassette was excised in this strain. This strain originated on a B6;129 bachground. |
| Strain description | B6;129S4-Gopc<tm1.1Tno>. Gopc, golgi associtated PDZ and coiled-coil motif containing that predominantly localized at trans-Golgi region, propose that play a role in vesicle transport from the Golgi. The Gopc deficient male mice are infertile that spermatozoa showed complete lack of acrosomes. This knockout mice are proved to be powerful tools for understanding the mechanisms underlying spermatogenesism, and valuable animal mode for human globozoospermia. |
| Colony maintenance | Sibling Mating (Heterozygote x Wild-type, Heterozygote) |
| References | Proc. Natl. Acad. Sci. USA, 99, 11211-11216 (2002). 12149515 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gopcgolgi associated PDZ and coiled-coil motif containing protein10Gopc<tm1.1Tno>targeted mutation 1.1, Tetsuo Noda2210402P09Rik, GOPC1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)10 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP10loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)10neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)10nls Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly ApolyA10poly A |
Ordering Information | |
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| Donor DNA | mouse GOPC genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli neo, E. coli lacZ, poly A, phage P1 loxP, herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 11211-11216 (2002). It is agreed by the parties hereto that any patent or other intellectual property right to any inventions to be made by or derived of the use by the RECIPIENT of the BIOLOGICAL RESOURCE shall be jointly owned by the RECIPIENT and the DEPOSITOR. The share in the ownership on such patents etc. shall be agreed between the RECIPIENT and the DEPOSITOR. In the event that the RECIPIENT desires to file patent applications for such inventions, the RECIPIENT shall obtain a prior written consent of the DEVELOPER, Dr. Tetsuo Noda before the RECIPIENT files such patent applications. The RECIPIENT should negotiate in good faith with the DEPOSITOR, or its representatives, to agree a fair and reasonable consideration to the DEPOSITOR in respect of the contribution to the said intellectual property rights afforded by the provision of the BIOLOGICAL RESOURCE hereunder. The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR. The RECIPIENT agrees to include the DEVELOPER, Dr. Tetsuo Noda as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. |
| Depositor | Tetsuo Noda (Japanese Foundation for Cancer Research) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Bizkarguenaga M, Gomez-Santos L, Madrid JF, Sáez FJ, Alonso E. Zona Pellucida sperm-binding protein 3 receptor distribution during Gopc-/- globozoospermic spermatogenesis. Microsc Res Tech 85(4) 1454-1464(2022) 34870349 |