Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Apr 19, 2019
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RBRC No. RBRC01260  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6;129(Cg)-Xist<tm2Sado>  
Former Common name Xist1loxGFP mice  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Donor strain (129X1/SvJ x 129S1/Sv)F1-Kitl+ via R1 ES cell line  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Takashi Sado, National Institute of Genetics at 2004. R1 ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6.  
Strain description Xist gene knockout mice (Xist<1loxGFP>). Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).  
Colony maintenance Heterozygote (female) x Wild-type (male) [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome X  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 2 Symbol Xist  
Symbol name inactive X specific transcripts  
Chromosome X  
Common name No Data  
Symbol description No Data  
 3 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome X  
Common name No Data  
Symbol description No Data  
 4 Symbol SA  
Symbol name Splice acceptor  
Chromosome X  
Common name No Data  
Symbol description No Data  
 5 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome X  
Common name No Data  
Symbol description No Data  
References No Data  
Sex-reversed somatic cell cloning in the mouse.
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT must inform the DEPOSITOR the research project using the
BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to
avoid the conflict of interest with the DEPOSITOR.
The RECIPIENT should contact the DEPOSITOR in the case of application for
any patents with the results from these mice.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)  
 2 Mouse of the Month Feb 2009  
 3 Genotyping protocol <PCR>  
Depositor Sado, Takashi (Medical institute of bioregulation, Kyushu University)  
Strain Status /
Availability
(Expected delivery)

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