Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC01267  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name 129(Cg)-Numb<tm1Zili/tm1.1Zili> Numbl<tm1Zili/tm1.1Zili>/SiecRbrc  
Former Common name Numb [-/loxP]; Numblike [-/FRTneoRFRTloxP]  
H-2 Haplotype No Data  
Donor strain 129/Sv via GS1 ES cell line  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Targeting of Numb: 1997, Olav Zilian, ISREC
Targeting of Numblike: 2001,Olav Zilian, ISREC  
Strain description Compound heterozygous mice conditionally targeted for Numb and Numblike (loxP) and some of them deleted for the other conditional allele. The selection marker in Numb (loxPneoRloxP) was deleted by Cre recombinase. However, this cassette is still present in the conditionally targeted allele of Numblike in these mice as a cassette flanked with FRT sites (FRTneoRFRT; [loxn eo]). In general, the purpose of the conditional alleles is to analyze the function of the Numb and Numblike proteins in mice after their conditional ablation upon expression of Cre recombinase. Parents heterozygous for a deleted allele of each gene allow to generate (1) compound homozygous null mutant embryos for the study of embryonic phenotypes or (2) adult mice homozygous for the deletion of Numblike.  
Colony maintenance Compound heterozygous mice are efficient breeders (Numb[loxP / -]; Numblike[loxP / -]). By contrast, mice homozygous for the deletion of Numblike have a small litter size and, hence, reproduce inefficiently. That problem of low breeding efficiency might rely on diminished fertility of he female homozygotes.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 12  
Common name neo; neomycin;  
Symbol description No Data  
 2 Symbol Numb  
Symbol name numb gene homolog (Drosophila)  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 5 Symbol Numbl  
Symbol name numb-like  
Chromosome 7  
Common name No Data  
Symbol description No Data  
 6 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 7  
Common name No Data  
Symbol description No Data  
 7 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 7  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 8 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 7  
Common name neo; neomycin;  
Symbol description No Data  
References Curr Biol. 2001 Apr 3;11(7):494-501.
J Immunol. 2007 Jun 1;178(11):6746-51.  
Multiple roles of mouse Numb in tuning developmental cell fates.
Multiple roles of mouse Numb in tuning developmental cell fates.
Research applications Cell Biology Research,
Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. (For targeted Numb: Olav Zillan et al. Current Biology. 11(7): 494-501; for targeted Numblike : the first publication describing this allele by the DEPOSITOR). This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE.
1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
3) Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application.  
Additional information
 1 No Data  
Depositor Zilian, Olav (Swiss Institute for Experimental Cancer Research)  Zilian, Olav 
Strain Status /
Availability
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