Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 19, 2019
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RBRC No. RBRC01268  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6;129-Numb<tm1Zili> Numbl<tm1Zili>/SiecRbrc  
Former Common name Numb [loxP/loxP]; Numblike [loxP/loxP]  
H-2 Haplotype No Data  
Donor strain 129/Sv via GS1 ES cell line  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Targeting of Numb: 1997, Olav Zilian, ISREC
Targeting of Numblike: 2001,Olav Zilian, ISREC  
Strain description Compound mice conditionally targeted for Numb and Numblike (loxP; for nomenclature of the alleles: lox) ; the selection marker in Numb (loxPneoRloxP) and Numblike (FRTneoRFRT) was deleted by Cre and Flp recombinase, respectively. The mice ready for shipment to RIKEN BRC carry at this point the conditional alleles over a non-targeted, wild-type allele [lox/+]; absence of the transgene FLPe should be reconfirmed in their progeny.
Purpose of these conditional alleles: functional analysis of the Numb and Numblike proteins in mice by their conditional ablaion through expression of Cre recombinase.  
Colony maintenance Efficient breeding is expected for these compound mice once homozygous for each conditionally targeted allele (Numb[lox / lox]; Numblike[lox / lox]) as they were efficient breeders when the compound homozygotes still carried FRTneoRFRT in both Numblike lox alleles.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Numb  
Symbol name numb gene homolog (Drosophila)  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 4 Symbol Numbl  
Symbol name numb-like  
Chromosome 7  
Common name No Data  
Symbol description No Data  
 5 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 7  
Common name No Data  
Symbol description No Data  
References Curr Biol. 2001 Apr 3;11(7):494-501.
J Immunol. 2007 Jun 1;178(11):6746-51.  
Multiple roles of mouse Numb in tuning developmental cell fates.
Multiple roles of mouse Numb in tuning developmental cell fates.
Research applications Cell Biology Research,
Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. (For targeted Numb: Olav Zillan et al. Current Biology. 11(7): 494-501; for targeted Numblike : the first publication describing this allele by the DEPOSITOR). This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE.
1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
3) Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application.  
Additional information
 1 No Data  
Depositor Zilian, Olav (Swiss Institute for Experimental Cancer Research)  Zilian, Olav 
Strain Status /
Availability
(Expected delivery)

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