Strain Data Sheet

RBRC01274

Strain Information

Image
BRC No.RBRC01274
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(MT1-Kit*VAL620ALA)1/Rbrc
Former Common namemt-KITV620A Tg-1 BDF1 x B6
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
black with white spot [a/a B/B C/C Tg/+]
Strain developmentDeveloped by Dr. Takahiro Kunisada, Gifu University School of Medicine in 2004. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. Transgenic mice were backcrossed to C57BL/6.
Strain descriptionB6;D2-Tg(MT1-kit,VAL620ALA). Transgenic mouse expresses the Val 620 Ala mutated Kit gene. Mice carrying the transgene show white spots on one fourth of the body hair. This transgenic mice are useful for the study of Kit gene function.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6JJcl)Hemizygous mice show white spots on one fourth of the body hair.Reproductive performance is good.
ReferencesJ. Invest. Dermatol., 126, 1111-1118 (2006). 16456533

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kitkit oncogeneUNKitmouse c-kit cDNA (Val 620Ala )mouse MT-I promoter, human growth hormone poly A, 5' and 3' flanking regions of mouse MT gene

Ordering Information

Donor DNAmouse c-kit cDNA (Val 620 Ala), mouse MT-I promoter, human growth hormone poly A, 5' and 3' flanking regions of mouse MT gene
Research applicationCancer Research
Cell Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT is requested to include the DEPOSITOR as a co-author in the first publication resulted by the use of the
BIOLOGICAL RESOURCE provided within two years after the deposition to the RIKEN BRC. After acceptance of the first paper the DEPOSITOR only requests the RECIPIENT to cite the first paper by the DEPOSITOR in any publication.
DepositorTakahiro Kunisada (Gifu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data