Strain Data Sheet
Data update: Aug 20, 2019
Health Report   PCR Protocol   Thawing Method
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  Mouse Marking
RBRC No. RBRC01298  
Type TransgeneCartagena  
Species Mus musculus  
Strain name CAT-Rho-K DN/5-18  
Former Common name No Data  
H-2 Haplotype No Data  
Background strain C57BL/6J  
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene containing CMV promoter and bovine Rho-K DN cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.  
Strain description These transgenic mice express a dominant-negative form for RhoA for Rho-kinase (Rho-K DN) by using the Cre-loxP system to suppress the activity of these signaling molecules in developing tissues. The signaling through Rho GTPase is important in the regulation of cellular processes such as cell morphology, cell adhesion, cell motility, and apoptosis. Copy number: line 3-1 (RBRC01294)_10 copies, line 5-18 (RBRC01298)_1 copy.  
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  Tg mice are viable and fertile.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Rock2  
Symbol name Rho-associated coiled-coil containing protein kinase 2 (bovine)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter CMV promoter, rabbit beta-globin intron, rabbit beta-globin poly A, SV40 early gene poly A  
 3 Symbol cat  
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References J Neurosci 2004 Apr 7;24(14):3480-8.  
Survival of developing motor neurons mediated by Rho GTPase signaling pathway through Rho-kinase.
Research applications Cre/loxP system,
Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neursci., 24, 3480-3488 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  
Additional information
 1 Lab HP (Japanese)  
 2 Mouse of the Month Jun 2005  
 3 Genotyping protocol <PCR>  
Depositor Kobayashi, Kazuto (Fukushima Medical University)  Kobayashi, Kazuto 
Strain Status /
(Expected delivery)

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