Strain Data Sheet
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Data update: May 20, 2019
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RBRC No. RBRC01347  
Type TransgeneCartagena   wks
Species Mus musculus  
Strain name B6.Cg-Tg(CAG-floxed neo-DT)03Osb  
Former Common name REP-DT  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Masaru Okabe, Genome Information Research Center, Osaka University in 2003. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. C57BL/6 and DBA/2 mixed background.  
Strain description CAG-DT conditional transgenic mice (REP-DT mice). The transgene consists of the CAG promoter and the diphtheria toxin A chain (DT) cDNA separated by a floxed neo cassette. When the floxed neo cassette is excised by crossing with Cre mice, DT is expressed. It has been reported that healthy but sterile mice due to a disruption of germ line cells are generated when the REP-DT mice were mated with testis specific cre mice (Prm1-cre). Other tissue/organ-specific expression patterns of the transgene are not clear (Caution: The gene expression pattern in transgenic mice can be different from it of the endogenous gene.)  
Colony maintenance Carrier x Carrier, Noncarrier [or Crossing to C57BL/6NCrSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol DT  
Symbol name diphtheria toxin A (DT-A)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome UN  
Common name neo; neomycin;  
Symbol description No Data  
References Biochem Biophys Res Commun. 2004 Aug 20;321(2):275-9.  
Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
Research applications Cre/loxP system,
General Purpose  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 321, 275-279 (2004).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.  
Additional information
 1 Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Okabe, Masaru (Osaka university)  Okabe, Masaru 
Strain Status /
Availability
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