Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC01351  
Type Targeted Mutation CongenicCartagena  
Species Mus musculus  
Strain name B6.129S-Grm2<tm1Nak>/NakRbrc  
Former Common name C57BL/6-Grm2 tm (Nov.2011), GluR2 KO, mGluR2 KO  
H-2 Haplotype No Data  
Donor strain 129S6/SvEvTac via EK.CCE ES cell line  
Background strain No Data  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Yokoi et al. (1996). A neomycin resistance cassette replaced 15.3-kb DNA fragment containing exon 2. The Grm2 deficient mice were produced by backcrossing to C57BL/6 strain for 20 times.  
Strain description mGluR2 (Grm2, G protein-coupled receptor, family C, group 1, member B) is widely dstributed both presynaptically and postsynaptically in varietry of neural cells. mGluR deficient mice have no behavioral abnormalities. But, a locomotor sensitization to cocaine administration was increased significantly. Moreover, significant impairment in motor coordination was detected. mGluR2 deficient mice were useful to clarify the rule in synaptic regulation of glutamatergic transmission in the neural network.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Grm2  
Symbol name G protein-coupled receptor, family C, group 1, member B  
Chromosome 9  
Common name 4930441L02Rik, Grm2, mGluR2  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 9  
Common name neo; neomycin;  
Symbol description No Data  
References Proc Natl Acad Sci U S A 2005 Mar 15;102(11):4170-5. Epub 2005 Mar 7.
Science. 1996 Aug 2;273(5275):645-7.  
Enhanced cocaine responsiveness and impaired motor coordination in metabotropic glutamate receptor subtype 2 knockout mice.
Enhanced cocaine responsiveness and impaired motor coordination in metabotropic glutamate receptor subtype 2 knockout mice.
Research applications Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science, 273, 645-647 (1996). Proc Natl Acad Sci USA, 102, 4170-4175 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT is required to obtain a prior written consent from the DEPOSITOR on use of the BIOLOGICAL RESOURCE.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Nakanishi, Shigetada (Kyoto University)  Nakanishi, Shigetada 
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