Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC01353  
Type Targeted Mutation and TransgeneCartagena   wks
Species Mus musculus  
Strain name C57BL/6-Tg(grm6-lacZ)35Kyo- grm6tm1Kyo  
Former Common name mGluR6-/-/lacZ+  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Shigetada Nakanishi, Kyoto University Faculty of Medicine. Tg: the transgene was injected into the pronuclei of B6D2F1 fertilized eggs. KO: CCE/EK.CCE ES cells derived from 129S/SvEv-Gpi1<c> were used to generate the mutant mice. C57BL/6 semicongenic.  
Strain description Double mutant mice with transgene and knockout allele. Grm6 (glutamate receptor, metabotropic 6) gene is suggested to be responsible for ON responses in both the rod and cone systems. The transgene is mGluR6 gene fused to the beta-galactosidase reporter gene. This strain is useful for investigating the promoter function of the cell-specific and developmentally regulated expression of mGluR6.  
Colony maintenance Tg: Carrier x Carrier; KO: Homozygote x Homozygote  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Grm6  
Symbol name glutamate receptor, metabotropic 6  
Chromosome 11  
Common name mGluR6  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 11  
Common name neo; neomycin;  
Symbol description No Data  
Promoter mouse mGluR6 promoter  
 3 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 11  
Common name No Data  
Symbol description E. coli beta galactosidase  
References Cell. 1995 Mar 10;80(5):757-65.
J Neurosci. 1997 May 1;17(9):3014-23.
J Neurosci. 1999 Apr 1;19(7):2568-79.  
Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Research applications General Purpose,
Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 19, 2568-2579 (1999). J. Neurosci., 17, 3014-3023 (1997). Cell, 80, 757-765 (1995).
The RECIPIENT is required to obtain a prior written consent from the DEPOSITOR on use of the BIOLOGICAL RESOURCE.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Nakanishi, Shigetada (Kyoto University)  Nakanishi, Shigetada 
Strain Status /
Availability
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