Strain Data Sheet
Data update: Aug 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC01385  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6;FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/McleRbrc  
Former Common name Transgenic mouse, PTR-H  
H-2 Haplotype No Data  
Background strain No Data  
 1 Appearance No Data  
Genotype No Data  
Strain development The expression construct was created by cloning cDNAs encoding the selected genes and the promoter into a plasmid vector. After verification of the construct by nucleotide sequencing, the transcriptional unit was cleaved out with restriction enzymes, purified with biochemical method and used for microinjection. The offspring were initially examined by PCR genotyping and in vitro expression of the marker, EGFP, after transfection with Tet-OFF expression vector (Clontech). The entire process took from 1999 to 2003.  
Strain description The transcriptional unit integrated into PTR-H consists of a dominant negative form of TGF-beta type II receptor gene and EGFP marker under a bidirectional tetracycline-responsive promoter. By crossing with another transgenic mouse that express tetracycline-inducible transcriptional activator, TGF-beta transduction pathway can be disrupted in a conditional manner.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Tgfbr2  
Symbol name transforming growth factor, beta receptor II  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter E. coli bidirectional TRE promoter (tetracycline responsive promoter)  
 2 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Fluorescent Proteins/lacZ System,
Tet system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Frugier, T., Kyoko, K., Matthaei, K.I. and McLennan, I.S. (2005). Transgenic mice carrying a tetracycline-inducible transforming growth factor beta receptor (T_RII). Genesis 42, 1-5.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor Koishi, Kyoko (University of Otago)  Koishi, Kyoko 
Strain Status /
(Expected delivery)

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