Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: May 20, 2019
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC01386  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6;FVB-Tg(ACTA1-cre)AMcle/Rbrc  
Former Common name Transgenic mouse, MuCre-A  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development The expression construct was created by cloning cDNAs encoding the selected genes and the promoter into a plasmid vector. After verification of the construct by nucleotide sequencing, the transcriptional unit was cleaved out with restriction enzymes, purified with biochemical method and used for microinjection. The offspring were initially examined by PCR genotyping. The in vivo expression was checked by crossing the mice with two Cre-reporter lines.  
Strain description MuCreA contains a transcriptional unit consisting of a muscle-specific promoter (alpha-actin promoter) and Cre recombinase gene. The mouse is used to make myogenic-specific knock-outs by crossing with mice that have a floxed gene. The MuCreA produces rapid and early cleavage of floxed genes from all differentiated myogenic cells, including cardiac myocytes and skeletal myotubes.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol No Data  
Symbol name SV40 poly A signal  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter human alpha actin promoter  
 2 Symbol cre  
Symbol name Phage P1 Cre recombinase  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Please cite Azhar, M, Wang, P.-Y., Frugier, T., Koishi, K., Deng, C., Noakes, P.G. and McLennan, I.S. (2010) Myocardial deletion of Smad4 using a novel alpha skeletal muscle actin Cre recombinase transgenic mouse causes misalignment of the cardiac outflow tract. International Journal of Biological Sciences 6: 546-555.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Koishi, Kyoko (University of Otago)  Koishi, Kyoko 
Strain Status /
Availability
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