Strain Information | |
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Image | |
BRC No. | RBRC01388 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129P2-Keap1<tm1Mym>/MymRbrc |
Former Common name | Keap1 knockout mice |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Masayuki Yamamoto, Center for Tsukuba Advanced Research Alliance, University of Tsukuba in 2003. Sequence encoding 8 through 204 of Keap1 was replaced with neomycin and nls-lacZ cassette. |
Strain description | Keap1 gene is a cytoplasmic protein, acts as a negative regulator of Nrf2 and as a sensor of xenobiotic and oxidative stresses. Double knockout mice of Nrf2 and Keap1 demonstrated that Nrf2 is a primary target of Keap1. Nrf2-Keap1 system is one of the major cellular protection mechanisms against xenobiotic and oxidative stresses. Keap1 homozygous mutant mice exhibit scaly skin, hyperkeratosis in the esophague and forestomatch, and died postnatally. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl]. Homozygous mutant mice are postnatal letha. |
References | Nat. Genet., 35, 238-45, (2003). 14517554 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)9 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)9neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)9nls |
Ordering Information | |
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Donor DNA | Simian virus 40 large T antigen nuclear localization signal (NLS), E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Keap1 genomic DNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Genetics, 35, 238-245 (2003).The DEPOSITOR should be a co-author in the articles when the users publish their data using these mice for 2 year after deposition to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice. |
Depositor | Masayuki Yamamoto (University of Tsukuba) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Mouse of the Month Oct 2005 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Sakai E, Morita M, Ohuchi M, Kido MA, Fukuma Y, Nishishita K, Okamoto K, Itoh K, Yamamoto M, Tsukuba T. Effects of deficiency of Kelch-like ECH-associated protein 1 on skeletal organization: a mechanism for diminished nuclear factor of activated T cells cytoplasmic 1 during osteoclastogenesis. FASEB J 31(9) 4011-4022(2017) 28515152 Chen H, Li J, Li H, Hu Y, Tevebaugh W, Yamamoto M, Que J, Chen X. Transcript profiling identifies dynamic gene expression patterns and an important role for Nrf2/Keap1 pathway in the developing mouse esophagus. PLoS One 7(5) e36504(2012) 22567161 Yin Y, Corry KA, Loughran JP, Li J. Moderate Nrf2 Activation by Genetic Disruption of Keap1 Has Sex-Specific Effects on Bone Mass in Mice. Sci Rep 10(1) 348(2020) 31941926 |