Strain Data Sheet
RBRC01389
Strain Information | |
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| Image |  |
| BRC No. | RBRC01389 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129P2-Ahr<tm1(AHR)Mym>/MymRbrc |
| Former Common name | Human AhR knock in mouse |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | C57BL/6J |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Dr. Takashi Moriguchi et al. in 2001. Human AHR cDNA and neomycin resistant gene were inserted in mouse Ahr gene. This construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. This knock-in mice were backcrossed with C57BL/6J for several times. |
| Strain description | The aryl hydrocarbon receptor (AhR) (dioxin receptor) is a transcription factor that mediates to catalyze xenobiotic Phase I metabolism. Human AhR knock in mouse, possessing human AHR instead of mouse Ahr, is useful as humanized mouse to predict the biological effects of bioaccumulative environmental toxicants like TCDD in humans. |
| Colony maintenance | Backcross to C57BL/6 (Heterozyote x C57BL/6JJ) |
| References | Proc. Natl. Acad. Sci. USA, 100, 5652-5657 (2003). 12730383 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ahraryl-hydrocarbon receptor12Ahr<tm1(AHR)Mym>targeted mutation 1, Masayuki YamamotoAh, Ahh, Ahre, dioxin receptor, In Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter AHRaryl-hydrocarbon receptor (human)12AHR Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)12neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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| Donor DNA | human arylhydrocarbon receptor AhR cDNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse arylhydrocarbon receptor AhR genomic DNA |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 100, 5652-5657 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Masayuki Yamamoto (University of Tsukuba) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |