Strain Data Sheet
Data update: Jul 19, 2019
Health Report   PCR Protocol   Thawing Method
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  Mouse Marking
RBRC No. RBRC01400  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6D2-Tg(CAG-Ptafr)1Tksh/TkshRbrc  
Former Common name PAFR-Tg, Platelet-activating factor (PAF) receptor transgenic mouse  
H-2 Haplotype No Data  
Background strain Crlj:B6D2F1  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Dr. Satoshi Ishii at the University of Tokyo in 1997. A transgenic construct containing a guinea-pig PAFR gene under the control of the a chiken beta actin promotor and the CMV immediate early enhancer, was introduced into the pronuclei of fertilized eggs of B6D2F2. The transgenic mice were mated with B6D2F1.  
Strain description Platelet-activating factor (PAF) is a phospholipid mediator with pleiotropic and potent biological effects on a variety of cells and tissues. This transgenic mice showed a bronchial hyperreactivity, increased endotoxin lethality and melanocytic tumorigenesis. This strain is useful to elucidating the pathophysiological roles of PAF and PAFR in vivo.  
Colony maintenance Cross to B6D2F1 (Hemizygote x Crj:B6D2F1)  
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Jul 2, 2007 2-検 D
May 8, 2007 2-検 D
May 7, 2007 2-検 D
Mar 5, 2007 2-検 D
Jan 9, 2007 2-検 D
Oct 30, 2006 2-検 D
Aug 29, 2006 2-検 D
Aug 28, 2006 2-検 D
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 1 Symbol Ptafr  
Symbol name platelet-activating factor receptor (guinea-pig)  
Chromosome UN  
Common name PAF receptor, PAFR  
Symbol description No Data  
References EMBO J 1997 Jan 2;16(1):133-42.  
Bronchial hyperreactivity, increased endotoxin lethality and melanocytic tumorigenesis in transgenic mice overexpressing platelet-activating factor receptor.
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
EMBO J., 16, 133-142 (1997).  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Shimizu, Takao (The University of Tokyo, Graduate School of Medicine)  Shimizu, Takao 
Strain Status /
(Expected delivery)

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