Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC01494  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6;129S2-Dppa3<tm1(rtTA)Nkn>/NknRbrc  
Former Common name PGC7 KO  
H-2 Haplotype No Data  
ES cell line D3 [129S2/SvPas]  
Donor strain 129S2/SvPas via D3 ES cell line  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Toshinobu Nakamura, Toru Kimura and Toru Nakano, Graduate School of Medicine and Frontier Biosciences, Osaka University in 2003. D3 ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6 (C57BL/6 semicongenic).  
Strain description Dppa3 (PGC7/Stella) gene knockout (rtTA knock-in mice). The start codon of Dppa3 was replaced with rtTA cDNA and floxed PGK-neo cassette. Dppa3 gene plays a critical role in embryonic development as a maternal facotor. Homozygote males mice are fully fertile, but homozygous females are infertile.  
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrSlc]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Dppa3  
Symbol name developmental pluripotency-associated 3  
Chromosome 6  
Common name 2410075G02Rik, PGC7, stella  
Symbol description No Data  
 2 Symbol rtTA  
Symbol name E. coli Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and the transactivation domain of VP-16)  
Chromosome 6  
Common name E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 6  
Common name neo; neomycin;  
Symbol description No Data  
References Unpublished  
Research applications Cre/loxP system,
Tet system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE.  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Nakamura, Toshinobu (Nagahama Institute of Bio-Science and Techology)  Nakamura, Toshinobu 
Strain Status /
Availability
(Expected delivery)

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