Strain Information | |
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Image | |
BRC No. | RBRC01519 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Tg(Thy1-spH)42Yyan/Rbrc |
Former Common name | TV-42, Thy1-SpH transgenic mice (TV-42) |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Yuchio Yanagawa, Gunma University Graduate School of Medicine and Hiromu Yawo, Tohoku University Graduate School of Life Sciences in 2002-2003. Transgene was injected into the introduced into the pronuclei of B6D2F2 fertilized eggs. The Tg mice were crossed to C57BL/6J. |
Strain description | Vesicular exocytosis at presynaptic terminals is fundamental function for neurotransmission and brain function. The lumen of a secretory vesicle is acidic (pH 5.6), whereas it becomes neutral (pH 7.4) instantaneously upon exocytosis. The intravesicular change of pH can be detected by a fluorescence change of a pH-sensitive derivative of green fluorescent protein (pHluorin). SynaptopHluorin (SpH) is a fusion protein of pHluorin and syntaxin (VAMP2) for targeting to secretory vesicles. Thy1-SpH transgenic mouse expresses SpH in brain under the control of neuron-specific Thy1.2 promoter. This strain is useful for studies of presynaptic terminals in various regions of the brain. The spH mRNAs was expressed in whole brains of TV-7, TV-10, and TV-34 transgenic lines, whereas in TV-42, it was expressed in the specific regions of the hippocampus. Tg mice mice show no obvious abnormality. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | J. Physiol. Sci., 59, 263-274 (2009). 19340534 Genesis 42, 53-60 (2005). 15880564 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter pHluorinpH-sensitive Green Fluorescent Protein (Aequorea victoria)UNpHluorin |
Ordering Information | |
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Donor DNA | murine Thy-1.2 expression cassette (Caroni, P. J. Neurosci. Methods 71, 3-9,1997), mouse vesicular membrane protein Vamp2 cDNA, Jellyfish pH-sensitive mutants GFP cDNA (pHluorin cDNA(Miesenbck, G., De Angelis, D.A., Rothman, J. E. Nature, 394, 192-195, 1998).) |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Araki, R., Sakagami, H., Yanagawa, Y., Hikima, T., Ishizuka, T., and Yawo, H. Transgenic mouse lines expressing synaptopHluorin in hippocampus and cerebellar cortex. Genesis 42, 53-60 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The Material should not be used for commercial purpose. |
Depositor | Hiromu Yawo (Tohoku University) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Sep 2006 |
BRC mice in Publications |
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No Data |