Strain Data Sheet
RBRC01577
Strain Information | |
|---|---|
| Image |  |
| BRC No. | RBRC01577 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C.Cg-Pik3r1<tm1Tka>/Rbrc |
| Former Common name | BALB/c-PI3K KO |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | BALB/cAnNCrlCrlj |
| Appearance | albino [A/A b/b c/c] |
| Strain development | Developed by Dr. Kadowaki of the University of Tokyo and backcrossed to BALB/c for 12 generations by depositor. |
| Strain description | C.Cg-Pik3r1<tm1Tka>. The PI3K, phosphoinositide-3-kinase gene is a key enzyme producing phospholipid second messengers and plays an important role in various signal transduction pathways. This knockout mouse strain is useful for investigating the function of the PI3K gene. |
| Colony maintenance | Since most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males. |
| References | Science, 283, 390-392 (1999). 9888854 Nat. Genet., 21, 230-235 (1999). 9988280 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |
|---|---|
| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pik3r1phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)13Pik3r1<tm1Tka>targeted mutation 1, Takashi Kadowakip50alpha, p55alpha, p85alpha, PI3K Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)13neo; neomycin; Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly AHSV TK polyA13poly A |
Ordering Information | |
|---|---|
| Donor DNA | mouse PI3K genomic DNA, E. coli neo, HSV TK polyA |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 283, 390-392 (1999). Nat. genet., 21, 230-235 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Shigeo Koyasu (Keio University) |
| Strain Status |  Frozen embryos |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
|---|
Umehara D, Watanabe S, Ochi H, Anai Y, Ahmed N, Kannagi M, Hanson C, Ruscetti S, Nishigaki K. Role of phosphatidylinositol 3-kinase in friend spleen focus-forming virus-induced erythroid disease. J Virol 84(15) 7675-82(2010) 20504929 |