Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC01601  
Type Targeted Mutation CongenicCartagena   wks
Species Mus musculus  
Strain name B6.129S7-Tert<tm1Fish>/FishRbrc  
Former Common name mTERT KO mice  
H-2 Haplotype No Data  
ES cell line JH1 [129S7/SvEvBrd]  
Donor strain 129S7/SvEvBrd via JH1 ES cell line  
Background strain C57BL/6NCrlCrlj  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Drs. Xunmei Yuan and Fuyuki Ishikawa at Tokyo Institute of Technology in 1999. A neomycin selection cassette replaced three exons, encoding a highly conserved motif A of the Tert gene. The mutant mice were backcrossed C57BL/6N.  
Strain description Tert, telomerase reverse transcriptase gene encodes a catalytic subunit of telomerase that is essential for telomerase activity. Homozygous mutant mice show no telomerase activity. Homozygous mutant mice after 3 to 5 generations were sterile.  
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)
Homozygous mutant mice after 3 to 5 generations were sterile.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Tert  
Symbol name telomerase reverse transcriptase  
Chromosome 13  
Common name EST2, TCS1, TP2  
Symbol description No Data  
 2 Symbol No Data  
Symbol name bovine growth hormone polyA signal  
Chromosome 13  
Common name No Data  
Symbol description No Data  
Promoter mouse RNA polymenase II promoter, bovine growth hormone poly A signal  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 13  
Common name neo; neomycin;  
Symbol description No Data  
References Genes Cells. 1999 Oct;4(10):563-72.  
Presence of telomeric G-strand tails in the telomerase catalytic subunit TERT knockout mice.
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genes to Cells, 4, 563-572 (1999).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The Recipient agrees to use the Biological Resource for academic use, shall not use for commercial use.  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Ishikawa, Fuyuki (Kyoto University)  Ishikawa, Fuyuki 
Strain Status /
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