Strain Information | |
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Image | |
BRC No. | RBRC01601 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.129S7-Tert<tm1Fish>/FishRbrc |
Former Common name | mTERT KO mice |
H-2 Haplotype | |
ES Cell line | JH1 [129S7/SvEvBrd] |
Background strain | C57BL/6NCrlCrlj |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Drs. Xunmei Yuan and Fuyuki Ishikawa at Tokyo Institute of Technology in 1999. A neomycin selection cassette replaced three exons, encoding a highly conserved motif A of the Tert gene. The mutant mice were backcrossed C57BL/6N. |
Strain description | Tert, telomerase reverse transcriptase gene encodes a catalytic subunit of telomerase that is essential for telomerase activity. Homozygous mutant mice show no telomerase activity. Homozygous mutant mice after 3 to 5 generations were sterile. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj) Homozygous mutant mice after 3 to 5 generations were sterile. |
References | Genes Cells, 4, 563-572 (1999). 10583505 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Terttelomerase reverse transcriptase13Tert<tm1Fish>targeted mutation 1, Fuyuki IshikawaEST2, TCS1, TP2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)13GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)13neo; neomycin;mouse RNA polymenase II promoter, bovine growth hormone poly A signal |
Ordering Information | |
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Donor DNA | mouse RNA polymenase II promoter, E. coli neo, bovine growth hormone polyA signal, mouse TERT genomic DNA |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes to Cells, 4, 563-572 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The Recipient agrees to use the Biological Resource for academic use, shall not use for commercial use. |
Depositor | Fuyuki Ishikawa (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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No Data |