Strain Data Sheet
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Data update: Apr 19, 2019
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RBRC No. RBRC01680  
Type Gene Trap   wks
Species Mus musculus  
Strain name B6.Cg-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>/JtakRbrc  
Former Common name Gene trap of Smoc1 (SPARC related modular calcium binding 1), PV384  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Dr. Junji Takeda at Osaka University in 2005.  
Strain description This strain is a gene trap strain that have an EGFP in Smoc1 gene, produced by using the SB transposon system.  
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 12  
Common name neo; neomycin;  
Symbol description No Data  
 2 Symbol Smoc1  
Symbol name SPARC related modular calcium binding 1  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 3 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 12  
Common name No Data  
Symbol description E. coli beta galactosidase  
 4 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome No Data  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 5 Symbol No Data  
Symbol name Mouse pgk promoter  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 6 Symbol No Data  
Symbol name Mouse synaptonemal protein 1 promoter  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 7 Symbol HBB2  
Symbol name rabbit beta-globin poly A  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 8 Symbol SA  
Symbol name Splice acceptor (Salmon)  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 9 Symbol SA  
Symbol name Splice acceptor (human bcl-2)  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 10 Symbol Splice donor  
Symbol name Sleeping Beauty transposase  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 11 Symbol Splice donor  
Symbol name Splice donor (Mouse hprt)  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
 12 Symbol poly A  
Symbol name human growth hormone poly adenylation signal sequence  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 13 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome No Data  
Common name No Data  
Symbol description No Data  
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.  
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Takeda Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Takeda, Junji (Osaka University)  Takeda, Junji 
Strain Status /
Availability
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