Strain Data Sheet

RBRC01683

Strain Information

Image
BRC No.RBRC01683
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pcdha<tm(CP)>/TyagRbrc
Former Common nameCNR CP
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2005. TT2 ES cells were used to generate the knockout mice.
Strain descriptionA polyA sequence in the CP exon of CNR (Pchda) was replaced with a neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pcdhaprotocadherin alpha cluster18Pcdha<tm(CP)>targeted mutationA830022B16Rik, Cnr7, Crnr7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)18neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAPhage P1 loxP, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Pcdha genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A.
Simultaneous detection of multiple transgenes for genetically-modified mouse strains.
Exp Anim 58(4) 437-42(2009) 19654444