Strain Data Sheet
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Data update: Jul 19, 2019
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RBRC No. RBRC01721  
Type TransgeneCartagena   wks
Species Mus musculus  
Strain name Bcl-xL Tg  
Former Common name No Data  
H-2 Haplotype No Data  
Background strain C57BL/6NCrlCrlj  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Dr. Gabriel Nunez at University of Michigan Medical School in 1995. The human Bcl-xL under the control of the SV40 promoter and IgH enhancer (SV40-EH casette) was injected into the pronuclei of (C57BL/6 x SJL/J)F2 fertilized egges.  
Strain description C57BL/6-Tg(SV40-BCL2L1)87Nnz. Bcl-xL, a member of the bcl-2 amily, is expressed in pre-B cells but downregulated at the immature and mature stages of B cell development. This transgenic mouse strain expresses the human Bcl-xL gene within B cell lineage under the control of the SV40 promoter and B IgH enhancer. The transgenic mice show marked accumulation of peripheral B cells in lymphoid organs and enhanced B cell survival in vitro.  
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL/6NCrj).  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol poly A  
Symbol name SV40 poly adenylation signal sequence  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter mouse Immunogloblin Eu enhancer, SV 40 early promoter, SV40 poly A additional signal  
 2 Symbol BCL2L1  
Symbol name Bcl2-like 1  
Chromosome UN  
Common name bcl-x, Bcl-XL, Bcl-Xs, BclX  
Symbol description No Data  
References J Exp Med. 1996 Feb 1;183(2):381-91.  
bcl-x exhibits regulated expression during B cell development and activation and modulates lymphocyte survival in transgenic mice.
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Exp. Med., 183, 381-391 (1991).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Timothy W. Behens).  
Additional information
 1 Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Koyasu, Shigeo (Keio University)  Koyasu, Shigeo 
Strain Status /
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