Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC01733  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6.129P2-Ptafr<tm1Tksh>/TkshRbrc  
Former Common name PAFR-KO, Platelet-activating factor (PAF) receptor knockout mouse  
H-2 Haplotype No Data  
ES cell line E14.1 [129P2/OlaHsd]  
Donor strain 129P2/OlaHsd via E14.1 ES cell line  
Background strain C57BL/6NCrlCrlj  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Dr. Satoshi Ishii at the University of Tokyo in 1998. A neomycin cassette was inserted into the open reading frame of Ptafr gene. The mutant mice were backcrossed to C57BL/6.  
Strain description Platelet-activating factor (PAF) is a phospholipid mediator with pleiotropic and potent biological effects on a variety of cells and tissues. Homozygous mutant mice showed decreased susceptibility to bacterial infection. This strain is useful to elucidating the pathophysiological roles of PAF and PAFR in vivo.  
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Ptafr  
Symbol name platelet-activating factor receptor  
Chromosome 4  
Common name PAF receptor, PAFR  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 4  
Common name neo; neomycin;  
Symbol description No Data  
References J Exp Med. 1998 Jun 1;187(11):1779-88.  
Impaired anaphylactic responses with intact sensitivity to endotoxin in mice lacking a platelet-activating factor receptor.
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Exp. Med., 187, 1779-1788 (1998).
The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Shimizu, Takao (The University of Tokyo, Graduate School of Medicine)  Shimizu, Takao 
Strain Status /
Availability
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