Strain Information | |
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Image | |
BRC No. | RBRC01765 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.129S4-Tob1<tm1Tya>/TyaRbrc |
Former Common name | tob-gene KO (B6) |
H-2 Haplotype | |
ES Cell line | J1 [129S4/SvJae] |
Background strain | C57BL/6 |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Tetsuo Noda at Japanese Foundation for Cancer Research Cancer Institute and Dr. Tadashi Yamamoto at the University of Tokyo in 1997. A PGK-neo cassette was inserted into the cording region of Tob1 gene. C57BL/6J background. |
Strain description | Tob is a member of antiproliferative protein family which is a negative regulator BMP/Smad signaling in osteoblasts. Tob1 null mice are viable and exhibit bone mass due to increased numbers of osteoblasts. This strain is useful for elucidating of Tob gene function. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) |
References | Genes Dev., 17, 1201-1206 (2003). 12756225 Cell, 103, 1085-1097 (2000). 11163184 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), mouse tob genomic DNA |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
Depositor | Tadashi Yamamoto (The University of Tokyo) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Schulze-Topphoff U, Casazza S, Varrin-Doyer M, Pekarek K, Sobel RA, Hauser SL, Oksenberg JR, Zamvil SS, Baranzini SE. Tob1 plays a critical role in the activation of encephalitogenic T cells in CNS autoimmunity. J Exp Med 210(7) 1301-9(2013) 23797093 Didonna A, Cekanaviciute E, Oksenberg JR, Baranzini SE. Immune cell-specific transcriptional profiling highlights distinct molecular pathways controlled by Tob1 upon experimental autoimmune encephalomyelitis. Sci Rep 6 31603(2016) 27546286 |