Strain Data Sheet

RBRC01768

Strain Information

Image
BRC No.RBRC01768
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;129-Zfp318<tm1Hhag>
Former Common nameTZF KO (B6), B6-TZK KO, B6;129-Zfp318<tm1>/Rbrc
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Hiromi Hagiwara, Faculty of Biomedical Engineering, Toin University of Yokohama in 2005.
Strain descriptionTZF (Zfp318) gene knockout mice. Homozygous males of C57BL/6 background (RBRC01768) have a tendency to exhibit infertile. Homozygous males of 129 background (RBRC01769) are infertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
ReferencesBiochem. Biophys. Res. Commun., 273, 398-403 (2000). 10873617
Proc. Natl. Acad. Sci. USA, 111, 4513-4518 (2014). 24616512
Mol. Cell. Endocrinology, 247, 150-165 (2006). 16469430
Biochem. Biophys. Res. Commun., 301, 1079-1085 (2003). 12589823
Biochem. Biophys. Res. Commun., 331, 1025-1031 (2005). 15882980

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Zfp318zinc finger protein 31817Zfp318<tm1Hhag>targeted mutation 1, Hiromi Hagiwara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)17

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAJellyfish GFP cDNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk poly A, mouse TZF genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorHiromi Hagiwara (Toin University of Yokohama)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

No Data