Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Aug 20, 2019
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RBRC No. RBRC01813  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6.Cg-Grin2a<tm1Mim>/MimRbrc  
Former Common name GluRε1 KO mouse  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Dr. Masayoshi Mishina at Tokyo University in 1995. The targeting vector containing pgk-neo cassette was transferred into TT2 ES cells to replace the exon encoding M2 and M3 transmembrane regions of Grin2a gene. The Grin2a deficient mice were backcrossed to C57BL/6N for 14 times.  
Strain description B6.Cg-Grin2a<tm1Mim>. The Grin2a gene is one of the glutamate receptor channel subunit families forming NMDA receptor channel, which is important for synaptic plasticity. The Grin2a homozygous mutant mice show a moderate deficiency in spatial learning. Reproductive performance and growth of them is normal.  
Colony maintenance backcross to C57BL/6 (Heterozygote x C57BL/6NJcl)  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Grin2a  
Symbol name glutamate receptor, ionotropic, NMDA2A (epsilon 1)  
Chromosome 16  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 16  
Common name neo; neomycin;  
Symbol description No Data  
References J Neurosci. 1998 Sep 1;18(17):6704-12.
  
Increased thresholds for long-term potentiation and contextual learning in mice lacking the NMDA-type glutamate receptor epsilon1 subunit.
Increased thresholds for long-term potentiation and contextual learning in mice lacking the NMDA-type glutamate receptor epsilon1 subunit.
Research applications Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 373, 151-155 (1995).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.  
Additional information
 1 Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine)  Mishina, Masayoshi 
Strain Status /
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