Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC01814  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6.Cg-Grin2b<tm1Mim>/MimRbrc  
Former Common name GluRε2 KO mouse  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Dr. Masayoshi Mishina at Tokyo University in 1995. A pgk-neo was transfered into TT2 ES cells to replace the exon encoding the transiation initiation site of Grin2b gene. The Mint deficient mice were backcrossed to C57BL/6N for 14 times.  
Strain description B6.Cg-Grin2b<tm1Mim>. The Grin2b gene is one of the glutamate recepoter channel subunit families forming NMDA receptor channel, which is important for synaptic plasticity. Homozygous mutant mice show impairments in suckling and die shortly after birth.  
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6NJcl)  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Grin2b  
Symbol name glutamate receptor, ionotropic, NMDA2B (epsilon 2)  
Chromosome 6  
Common name GluRepsilon2, NMDAR2B, Nmdar2b, NR2B  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 6  
Common name neo; neomycin;  
Symbol description No Data  
References Eur J Neurosci. 2001 Jul;14(1):153-60.
Neuron. 1996 Feb;16(2):333-44.  
Roles of the glutamate receptor epsilon2 and delta2 subunits in the potentiation and prepulse inhibition of the acoustic startle reflex.
Roles of the glutamate receptor epsilon2 and delta2 subunits in the potentiation and prepulse inhibition of the acoustic startle reflex.
Research applications Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neuron, 16 ,333-344 (1996).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.  
Additional information
 1 Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine)  Mishina, Masayoshi 
Strain Status /
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