Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC01891  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129-Clec10a<tm1Hed>/TatsRbrc  
Former Common name mMgl1 knockout mice (C57BL/6J)  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Background strain C57BL/6J  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Stephen M Hedrick, University of California. R1 ES cells were used to generate the knockout mice. R RBRC01891: backcrossed to C57BL/6J over 8 times. RBRC01892: backcrossed to C57BL/6J for 8 times, and then backcrossed to BALB/c over 6 times.  
Strain description Mgl1 gene knockout mice. Exons 2 and 3 were replaced with a PGK-neo cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. C57BL/6 background (RBRC01891) and BALB/c mixed background (RBRC01892). Homozygous mutant mice were viable and fertile.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Clec10a  
Symbol name macrophage galactose N-acetyl-galactosamine specific lectin 1  
Chromosome 11  
Common name Mgl1  
Symbol description No Data  
 2 Symbol GH  
Symbol name Growth hormone polyA (Bovine)  
Chromosome 11  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 11  
Common name neo; neomycin;  
Symbol description No Data  
References Mol Cell Biol. 2002 Jul;22(14):5173-81.  
Generation of mice deficient for macrophage galactose- and N-acetylgalactosamine-specific lectin: limited role in lymphoid and erythroid homeostasis and evidence for multiple lectins.
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.  
Additional information
 1 Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Irimura, Tatsuro (The University of Tokyo)  Irimura, Tatsuro 
Strain Status /
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