Strain Information | |
---|---|
Image | |
BRC No. | RBRC01892 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C.B6(129)-Clec10a<tm1Hed>/TatsRbrc |
Former Common name | mMgl1 knockout mice (BALB/c) |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | BALB/cAnNCrlCrlj |
Appearance | |
Strain development | Developed by Stephen M Hedrick, University of California. R1 ES cells were used to generate the knockout mice. R RBRC01891: backcrossed to C57BL/6J over 8 times. RBRC01892: backcrossed to C57BL/6J for 8 times, and then backcrossed to BALB/c over 6 times. |
Strain description | Mgl1 gene knockout mice. Exons 2 and 3 were replaced with a PGK-neo cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. C57BL/6 background (RBRC01891) and BALB/c mixed background (RBRC01892). Homozygous mutant mice were viable and fertile. |
Colony maintenance | Homozygote x Homozygote [or Crossing to BALB/cAnNCrlCrlj] |
References | Mol. Cell. Biol., 22, 5173-5181 (2002). 12077344 |
Health Report | |
---|---|
Examination Date / Room / Rack | 2024/02/26Room:4-BRack:I |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Clec10amacrophage galactose N-acetyl-galactosamine specific lectin 111Clec10a<tm1Hed>targeted mutation 1, Stephen M HedrickMgl1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)11GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
---|---|
Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine growth hormone poly A, mouse Clec10a genomic DNA |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
Depositor | Tatsuro Irimura (The University of Tokyo) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |