Strain Information | |
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Image | |
BRC No. | RBRC01897 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Sox2<tm1Okud> |
Former Common name | Floxed Sox2 mice |
H-2 Haplotype | |
ES Cell line | E14TG2a [129P2/OlaHsd] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Akihiko Okuda, Research Center for Genomic Medicine, Saitama Medical University in 2004. C57BL/6 background. |
Strain description | Sox2 gene is a member of the SRY-related HMG box gene family that encode transcription factors with a single HMG DNA-binding domain, expresses in multipotent neural stem cells at all stages of mouse ontogeny. Sox2 ORF was replaced with IREShygTKpA cassette flanked by loxP sites. Conditional Sox2 deficient mice can be generated by crossing this mice with tissue-specific Cre mice. |
Colony maintenance | Heterozygote (female) x Wild-type (male) [C57BL/6JJcl]. Fertility of heterozygous male is low. |
References | FEBS Lett., 582, 2811-2815 (2008). 18638478 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)3internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, Encephalomyocorditis virus (EMCV) internal ribosomal entrysite (IRES), E. coli hygromycin phosphotransferase, herpes simplex virus thymidine kinase polyA, mouse Sox2 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. |
Depositor | Akihiko Okuda (Saitama Medical University) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) |
Additional Info. | Mouse of the Month Sep 2009 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |