Strain Data Sheet

RBRC01903

Strain Information

Image
BRC No.RBRC01903
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Lats2<tm1Noj>
Former Common nameTats2 heterozygous (He) mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Norikazu Yabuta and Hiroshi Nojima, Research Institute for Microbial Diseases, Osaka University in 2002. D3 ES cells were used. The mutant mice were crossed to C57BL/6.
Strain descriptionLats2 gene knockout mice. The first coding exon was replaced with a PGK-neo cassette. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
ReferencesJ Biol Chem., 282, 19259-19271 (2007). 17478426

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lats2large tumor suppressor 214Lats2<tm1Noj>targeted mutation 1, Hiroshi Nojima

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse Lats2 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationCell Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Biol Chem., 282, 19259-19271 (2007).The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorHiroshi Nojima (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data