Strain Data Sheet
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Data update: Jul 19, 2019
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RBRC No. RBRC01918  
Type TransgeneCartagena  
Species Mus musculus  
Strain name B6.Cg-Tg(deRFP)2Rbrc  
Former Common name Oct-delta PE-mRFP#2, TgN(de RFP)2Rbrc  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Kuniya Abe, RIKEN BioResrouce Center in 2004. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mutant mice were crossed to C57BL/6 over N2 times.  
Strain description Oct-4/RFP transgenic mice carry mRFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) by deletion in the proximal enhancer (PE). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express red fluorescent protein at various stages of embryonic development.  
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  
Health Report No Data  
Gene Details
Promoter Oct 3/4 promoter  
 1 Symbol mRFP  
Symbol name monomeric Red Fluorescent Protein (Discosoma sp.)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Unpublished  
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using these mice for 1 year after provided to the RIKEN BRC. Users should be contact the DEPOSITOR in the case of application for any patents with the results from these mice.  
Additional information
 1 Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Abe, Kuniya (RIKEN, BioResource Center)  Abe, Kuniya 
Strain Status /
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