Strain Data Sheet
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Data update: Jul 19, 2019
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RBRC No. RBRC01922  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6;129-Zeb1<tm1Yhi>  
Former Common name δ EF1 [Delta]C-fin, δ EF1 [Delta]C727  
H-2 Haplotype No Data  
ES cell line E14 [129P2/OlaHsd]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Yujiro Higashi, Institute for Molecular and Cellular Biology, Osaka University. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 11 times.  
Strain description Zeb1 gene knockout mice (deltaEF1 deltaC727). Exon 6 of the Zeb1 was replaced with a neo cassette. In the genetic background of inbred mouse strains, homozygous mutant mice die after birth. While about 20-30% of homozygous mice survive to adulthood in the mixed genetic background.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Zfhx1a  
Symbol name zinc finger homeobox 1a  
Chromosome 18  
Common name [delta]EF1, 3110032K11Rik, AREB6, MEB1, Nil2, Nil2, Tcf18, Tcf8, ZEB, Zfhep, Zfx1a  
Symbol description No Data  
Promoter SV40 early promoter and herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 18  
Common name neo; neomycin;  
Symbol description No Data  
References J Exp Med. 1997 Apr 21;185(8):1467-79.  
Impairment of T cell development in deltaEF1 mutant mice.
Research applications Developmental Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Exp. Med., 185, 1467-1480 (1997).
The RECIPIENT should consult with the DEPOSITOR in the case of application for any patents or commercial use with the results derived from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Higashi, Yujiro (Graduate School of Frontier Biosciences, Osaka University)  Higashi, Yujiro 
Strain Status /
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