Strain Data Sheet
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Data update: Apr 19, 2019
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RBRC No. RBRC01949  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6;129S4(Cg)-Tsix<tm1Sado>  
Former Common name Tsix SA; Tsix splicing defect mutant mice  
H-2 Haplotype No Data  
ES cell line J1 [129S4/SvJae]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Takashi Sado, National Institute of Genetics in 2006. J1 ES cells were used to generate the mutant mice. Puromycin resistance was excised by crossing with cre transgenic mice.  
Strain description Tsix gene splicing knockout mice (Tsix<SA>). The splicing acceptor site of Tsix exon 4 was replaced with a fragment containing an IRES-EGFP and a floxed puromycin resistance gene. This mutant line carries modified allele of Tsix locus to avoid the production major splicing variant. Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).  
Colony maintenance Heterozygote (female) x Wild-type (male) [C57BL/6JJcl]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome X  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 2 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome X  
Common name No Data  
Symbol description No Data  
 3 Symbol Tsix  
Symbol name X (inactive)-specific transcript, antisense  
Chromosome X  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome X  
Common name No Data  
Symbol description No Data  
References Development. 2006 Dec;133(24):4925-31. Epub 2006 Nov 15.  
Tsix defective in splicing is competent to establish Xist silencing.
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development, 133, 4925-4931 (2006).
The RECIPIENT must inform the DEPOSITOR the research project using the
BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to
avoid the conflict of interest with the DEPOSITOR.
The RECIPIENT should contact the DEPOSITOR in the case of application for
any patents with the results from these mice.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)  
 2 Mouse of the Month Feb 2009  
 3 Genotyping protocol <PCR>  
Depositor Sado, Takashi (Medical institute of bioregulation, Kyushu University)  
Strain Status /
Availability
(Expected delivery)

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