Strain Data Sheet
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Data update: Apr 19, 2019
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RBRC No. RBRC01950  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6;129S4-Xist<tm1Sado> Tsix<tm1Enl>  
Former Common name X; Xist/Tsix double KO mouse  
H-2 Haplotype No Data  
ES cell line J1 [129S4/SvJae]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Takashi Sado, National Institute of Genetics in 2005.  
Strain description Xsit/Tsix double knockout mice (X<dc>). An IRES-EGFP cassette was inserted to replace part of exon 1 of Xist gene in mutated J1 ES cells lacking Tsix gene (Tsix<tm1Enl>) in which exon 2 and 3 were replaced with an IRES Beta-geo cassette. Heterozygous mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).  
Colony maintenance Heterozygote (female) x Wild-type (male) [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome X  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 2 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome X  
Common name neo; neomycin;  
Symbol description No Data  
 3 Symbol Xist  
Symbol name inactive X specific transcripts  
Chromosome X  
Common name No Data  
Symbol description No Data  
 4 Symbol Tsix  
Symbol name X (inactive)-specific transcript, antisense  
Chromosome X  
Common name No Data  
Symbol description No Data  
 5 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome X  
Common name No Data  
Symbol description No Data  
 6 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome X  
Common name No Data  
Symbol description E. coli beta galactosidase  
 7 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome X  
Common name No Data  
Symbol description No Data  
References Dev Cell. 2005 Jul;9(1):159-65.  
Tsix silences Xist through modification of chromatin structure.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Cell, 9, 159-165 (2005).
The RECIPIENT must inform the DEPOSITOR the research project using the
BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to
avoid the conflict of interest with the DEPOSITOR.
The RECIPIENT should contact the DEPOSITOR in the case of application for
any patents with the results from these mice.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)  
 2 Mouse of the Month Feb 2009  
 3 Genotyping protocol <PCR>  
Depositor Sado, Takashi (Medical institute of bioregulation, Kyushu University)  
Strain Status /
Availability
(Expected delivery)

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