Strain Information | |
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Image | |
BRC No. | RBRC01952 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129S2-Lats1<tm1Noj> |
Former Common name | Lats1 heterozygous (He) mouse |
H-2 Haplotype | |
ES Cell line | D3 [129S2/SvPas] |
Background strain | |
Appearance | |
Strain development | Developed by Norikazu Yabuta and Hiroshi Nojima, Research Institute for Microbial Diseases, Osaka University in 2004. D3 ES cells were used. The mutant mice were crossed to C57BL/6. |
Strain description | Lats1 gene knockout mice (deltaN type). Exon 1 containing a translation initiation codon was replaced with a PGK-neo cassette. Homozygous mutant mice are small. Homozygous mutant females cannot nurse pups due to lacking mammae. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NCrSlc] |
References | J. Cell Sci., 126, 508-520 (2013). 23230145 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)10neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Lats1 genomic DNA |
Research application | Cell Biology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J CellSci., 126, 508-520 (2013).The RECIPIENT agrees to use of this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
Depositor | Hiroshi Nojima (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |