Strain Data Sheet

RBRC01959

Strain Information

Image
BRC No.RBRC01959
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Blm<tm1Jtak>
Former Common name129-Blmtm1Tak
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain129S6/SvEvTac
Appearance
Strain developmentDeveloped by Junji Takeda at Osaka University in 2001. A cassette containing a tetracycline-controlled transactivatior (tTA), and a tetracycline responsive element (TRE) was insertied in exon 2. The mutant mice were mated with 129S6/SvEvTac and then maintained by sister-brother mating.
Strain description
Colony maintenance
ReferencesNature, 429, 896-899 (2004). 15215867

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BlmBloom syndrome homolog (human)7Blm<tm1Khor>targeted mutation 1, Kyoji Horie

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;Herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)7tTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain

Ordering Information

Donor DNAE. coli Tetraycline operator, E. coli Tetracyline repressor, E. coli Neomycin resistance gene, Herpes simplex virus VP16 gene, Herpes simplex virus Thymidine kinase promoter, Herpes simplex virus Thymidine kinase polyA signal, P1 phage LoxP, Adenovirus Tripartite leader sequeence, SV40 PolyA signal, Bovine Growth hormone polyA signal
Research applicationCre/loxP system
Tet system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorJunji Takeda (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Takeda Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data