Strain Data Sheet
RBRC01967
Strain Information | |
|---|---|
| Image |  |
| BRC No. | RBRC01967 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129S4-Nanog<tm1Yam> |
| Former Common name | |
| H-2 Haplotype | |
| ES Cell line | RF8 [129S4/SvJae] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exson 2 whcich contains the homeodomain. The mutant mice were backcross to C57BL/6J. |
| Strain description | Nanog gene is a important factor of maintaining ES cell pluripotent differentiation.Homozygous mutant mice are embryonic lethal. |
| Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) |
| References | Cell, 113, 631-642 (2003). 12787504 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)6internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin; |
Ordering Information | |
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| Donor DNA | mouse Nanog genomic DNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 poly A |
| Research application | Developmental Biology Research |
| Specific Term and Conditions | |
| Depositor | Shinya Yamanaka (Kyoto University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |