Strain Data Sheet

RBRC01968

Strain Information

Image
BRC No.RBRC01968
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Visfatin<tm1Yam>
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2001. The mutant mice were backcross to C57BL/6J.
Strain descriptionVisfatin is produced by the visceral fat and whose expression level in plasma increases during the development of obesity. Visfatin exterted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. Homozygous mutant mice are embryonic lethal. Heterozygous mice show increased plasma glucose levels but normal insulin evels.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Namptnicotinamide phosphoribosyltransferase12Nampt<tm1Yam>targeted mutation 1, Shinya Yamanaka1110035O14Rik, Visfatin, Pbef1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)12En2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)12internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)12

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)12neo; neomycin;

Ordering Information

Donor DNAEn2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocorditis virus (EMCV) internal ribosomal entrysite (IRES), E. coli neo, E. coli lacZ, SV40 poly A, mouse Visfatin genomic DNA
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall conclude the Memorandum of Understanding with KYOTO UNIVERSITY (contact KYOTO UNIVERSITY at cira-keiyaku@cira.kyoto-u.ac.jp) before receiving the ORIGINAL MATERIAL.
DepositorShinya Yamanaka (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data