Strain Data Sheet
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC01968  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129S4-Visfatin<tm1Yam>  
Former Common name No Data  
H-2 Haplotype No Data  
ES cell line RF8 [129S4/SvJae]  
Background strain C57BL/6JJcl  
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2001. The mutant mice were backcross to C57BL/6J.  
Strain description Visfatin is produced by the visceral fat and whose expression level in plasma increases during the development of obesity. Visfatin exterted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. Homozygous mutant mice are embryonic lethal. Heterozygous mice show increased plasma glucose levels but normal insulin evels.  
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Nampt  
Symbol name nicotinamide phosphoribosyltransferase  
Chromosome 12  
Common name 1110035O14Rik, Visfatin, Pbef1  
Symbol description No Data  
 2 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome 12  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 12  
Common name neo; neomycin;  
Symbol description No Data  
 4 Symbol En2  
Symbol name En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)  
Chromosome 12  
Common name No Data  
Symbol description No Data  
 5 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 12  
Common name No Data  
Symbol description E. coli beta galactosidase  
References No Data  
Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT shall conclude the Memorandum of Understanding with KYOTO UNIVERSITY (contact KYOTO UNIVERSITY at before receiving the ORIGINAL MATERIAL.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Yamanaka, Shinya (Institute for frontier medical sciences, Kyoto university)  Yamanaka, Shinya 
Strain Status /
(Expected delivery)

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