Strain Information | |
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Image | |
BRC No. | RBRC02032 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6;B6C3-Tg(CAG/Acr-EGFP)CX-FM139Osb |
Former Common name | B6C3F1-Tg(CAG/Acr-EGFP)CX-FM139Osb (Nov. 2011), B6D2F1-Tg(CAG-EGFP)CX-FM139Osb |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | black [a/a b/b C/C] |
Strain development | Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. This transgenic line was generated by coinjected with act-EGFP and acr-EGFP transgene constructs into fertilized eggs. The mice were crossed to C57BL/6. |
Strain description | Transgenic mice expressing GFP ubiquitously from CAG-EGFP and in the sperm acrosome from acr3-EGFP. GFP expression is detected in germline cells at early stage (8 cells). Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG-EGFP, in which EGFP is connected to the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG). The transgene is located in chromosome X by FISH. |
Colony maintenance | Carrier x Noncarrier [C57BL/6NJcl] |
References | Genomics, 80(6):564-574 (2002). 12504848 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)XCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Ordering Information | |
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Donor DNA | CX-EGFP (Jellyfish GFP cDNA, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, rabbit beta-globin polyadenylation signal |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genomics, 80(6):564-574 (2002).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masaru Okabe (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |