Strain Data Sheet
Data update: Aug 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC02032  
Type TransgeneCartagena   wks
Species Mus musculus  
Strain name B6;B6C3-Tg(CAG/Acr-EGFP)CX-FM139Osb  
Former Common name B6C3F1-Tg(CAG/Acr-EGFP)CX-FM139Osb (Nov. 2011), B6D2F1-Tg(CAG-EGFP)CX-FM139Osb  
H-2 Haplotype No Data  
Background strain No Data  
 1 Appearance black  
Genotype a/a b/b C/C  
Strain development Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. This transgenic line was generated by coinjected with act-EGFP and acr-EGFP transgene constructs into fertilized eggs. The mice were crossed to C57BL/6.  
Strain description Transgenic mice expressing GFP ubiquitously from CAG-EGFP and in the sperm acrosome from acr3-EGFP. GFP expression is detected in germline cells at early stage (8 cells). Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG-EGFP, in which EGFP is connected to the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG). The transgene is located in chromosome X by FISH.  
Colony maintenance Carrier x Noncarrier [C57BL/6NJcl]  
Health Report
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 1 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome X  
Common name No Data  
Symbol description No Data  
References Genomics. 2002 Dec;80(6):564-74.
Proc Natl Acad Sci U S A 2005 Mar 15;102(11):4039-44. Epub 2005 Mar 3.
FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University ( The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Okabe, Masaru (Osaka university)  Okabe, Masaru 
Strain Status /
(Expected delivery)

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