Strain Information | |
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Image | |
BRC No. | RBRC02097 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | STOCK Izumo1<tm1Osb> |
Former Common name | Izumo KO |
H-2 Haplotype | |
ES Cell line | D3 [129S2/SvPas] |
Background strain | |
Appearance | |
Strain development | Developed by Drs. Naokazu Inoue and Masaru Okabe at Osaka University in 2003. 129, C57BL/6 and DBA/2 mixed background. |
Strain description | Izumo1 is a immunoglobulin super family protein that is required for sperm to fuse with eggs. Homozygous mutant mice are viable but males are sterile. The Izumo-deficient sperm can bind to an egg, and even burrow through the zona pellucida, but are incapable of fusing with egg. |
Colony maintenance | Sibling Mating (Hemizygote x Wild-type, Hemizygote) Homozygous mutant males are sterile. |
References | Nature, 434, 234-238 (2005). 15759005 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Izumo1izumo sperm-egg fusion 17Izumo1<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University1700058F15Rik Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, mouse PGK promoter, E. coli neo, mouse Izumo1 genomic DNA |
Research application | Cre/loxP system Reproductive Biology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 434, 234-238 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masaru Okabe (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |